Location of Sampling Stations: Shane Creek (T-9) Frequency of Sampling: Base flow samples are collected 3 times per week in mornings. If the base flow collection time corresponds to a storm flow event, they are classified as such. Variable Measured: NO3-N+ NO2-N, NH4-N, total-N, SRP (or soluble reactive phosphate), total-P, and dissolved organic carbon. Field Methods: Samples (250 mL) from mid-stream are collected 2-3 times per week. Date, time of day (CST), stream temperature (c) and stream height. Sub-samples (three 20mL vials) are taken upon returning to the lab. Samples are immediately frozen. Laboratory Methods: One vial is analyzed for inorganic nitrogen (N) and phosphorous (P). A second vial is run for dissolved organic carbon (DOC). The third vial is run for TN or total nitrogen, and TP or total phosphorous. At the completion of all analyses the vial with maximum volume is stored in the freezer as an archived sample for 10 years from date of collection. Nitrogen and phosphorus nutrients are performed by colorimetric determination on a flow solution analyzer. Dissolved organic carbon is measured by high temperature combustion. Data is compiled annually, checked by the RA, and archived by the IM. For additional metadata information see: http://lter.konza.ksu.edu/sites/default/files/DC.pdf For additional methods information see: http://lter.konza.ksu.edu/sites/default/files/MM.pdf