02400nas a2200277   4500008004100000245008900041210006900130300001300199490000800212520152400220653000901744653002301753653001401776653002301790653002601813653002601839653002601865653001801891653001601909653001901925100001701944700001701961700001501978700002001993856010902013       2004                        eng d00aInterfertility of two mating populations in the Gibberella fujikuroi species complex0 aInterfertility of two mating populations in the Gibberella fujik  a611 -6180 v1103 aGibberella fujikuroi and Gibberella intermedia (mating populations ‘C’ and ‘D’ of the G. fujikuroi species complex) can be distinguished by differences in the spectrum of mycotoxins produced, the lack of sexual cross-fertility and diagnostic differences in their DNA sequences. Some isolates from these two biological species, however, can interbreed and complete meiosis to produce viable progeny. Analysis of marker segregation amongst such hybrid progeny can be used to estimate the degree of genomic rearrangement and genetic incompatibility that has accumulated since these sibling species diverged. Recombinant progeny were isolated from crosses of the standard tester strains for these two species and from crosses between these standard testers and a field isolate (KSU X-10626) that was cross-fertile with tester strains of both species. Progeny in all of the crosses segregated for amplified fragment length polymorphisms (AFLPs). Segregation of AFLP loci deviated from 1:1 for two thirds of the loci amongst the progeny of the cross between the ‘C’ and ‘D’ mating population tester strains, but <20% of the polymorphic loci in the cross of either tester with KSU X-10626 showed such distortion. It was concluded that G. intermedia and G. fujikuroi are sufficiently interfertile to belong to the same biological species, but that changing the nomenclature to reflect this interfertility requires more evidence for the natural occurrence of a continuum in fertility than is presently available.10aAFLP10abiological species10afumonisin10aFusarium fujikuroi10aFusarium proliferatum10aGibberella intermedia10ainterspecific hybrids10aKonza Prairie10amating type10asexual crosses1 aLeslie, J.F.1 aZeller, K.A.1 aWohler, M.1 aSummerell, B.A.  uhttp://129.130.186.12/content/interfertility-two-mating-populations-gibberella-fujikuroi-species-complex