02384nas a2200205   4500008004100000245012100041210006900162300001300231490000800244520163800252653003401890653001201924653001701936653002701953653001801980100001801998700001702016700001802033856012702051       2010                        eng d00aIsolation and morphological and metabolic characterization of common endophytes in annually burned tallgrass prairie0 aIsolation and morphological and metabolic characterization of co  a813 -8210 v1023 aDark septate endophytes (DSE) are common and abundant root-colonizing fungi in the native tallgrass prairie. To characterize DSE fungi were isolated from roots of mixed tallgrass prairie plant communities. Isolates were grouped according to morphology, and the grouping was refined by ITS-RFLP and/or sequencing of the ITS region. Sporulating species of Periconia, Fusarium, Microdochium and Aspergillus were isolated along with many sterile fungi. Leek resynthesis was used to quickly screen for DSE fungi among the isolates. Periconia macrospinosa and Microdochium sp. formed typical DSE structures in the roots; Periconia produced melanized intracellular microsclerotia in host root cortex, whereas Microdochium produced abundant melanized inter- and intracellular chlamydospores. To further validate the results of the leek resynthesis growth responses of leek and a dominant prairie grass, Andropogon gerardii, were assessed in a laboratory resynthesis system. Leek growth mainly was unresponsive to the inoculation with Periconia or Microdochium, whereas Andropogon tended to respond positively. Select Periconia and Microdochium isolates were tested further for their enzymatic capabilities and for ability to use organic and inorganic nitrogen sources. These fungi tested positive for amylase, cellulase, polyphenol oxidases and gelatinase. Periconia isolates used both organic and inorganic nitrogen sources. Our study identified distinct endophytes in a tallgrass prairie ecosystem and indicated that these endophytes can use a variety of complex nutrient sources, suggesting facultative biotrophic and saprotrophic habits.10aDark septate endophytes (DSE)10aenzymes10aMicrodochium10aPericonia macrospinosa10asterile fungi1 aMandyam, K.G.1 aLoughlin, T.1 aJumpponen, A.  uhttp://129.130.186.12/content/isolation-and-morphological-and-metabolic-characterization-common-endophytes-annually-burned