@article {KNZ001335,
	title = {Divergent host plant adaptation drives the evolution of reproductive  isolation in the grasshopper Hesperotettix viridis (Orthoptera: Acrididae)},
	journal = {Biological Journal of the Linnaean Society},
	volume = {100},
	year = {2010},
	pages = {866 -878},
	abstract = {<p>Early stages of lineage divergence in insect herbivores are often related to shifts in host plant use and divergence in mating capabilities, which may lead to sexual isolation of populations of herbivorous insects. We examined host preferences, degree of differentiation in mate choice, and divergence in cuticular morphology using near-infrared spectroscopy in the grasshopper Hesperotettix viridis aiming to understand lineage divergence. In Kansas (USA), H. viridis is an oligophagous species feeding on Gutierrezia and Solidago host species. To identify incipient mechanisms of lineage divergence and isolation, we compared host choice, mate choice, and phenotypic divergence among natural grasshopper populations in zones of contact with populations encountering only one of the host species. A significant host-based preference from the two host groups was detected in host-paired feeding preference studies. No-choice mate selection experiments revealed a preference for individuals collected from the same host species independent of geographic location, and little mating was observed between individuals collected from different host species. Female mate choice tests between males from the two host species resulted in 100\% fidelity with respect to host use. Significant differentiation in colour and cuticular composition of individuals from different host plants was observed, which correlated positively with host choice and mate choice. No evidence for reinforcement in the zone of contact was detected, suggesting that divergent selection for host plant use promotes sexual isolation in this species.</p>},
	keywords = {LTER-KNZ, allopatry, ecological speciation, feeding preference, genetic divergence, host-associated divergence, mate choice, Microsatellites, near-infrared spectroscopy, reproductive isolation, secondary contact zones, sexual isolation, sympatry},
	doi = {10.1111/j.1095-8312.2010.01458.x},
	url = {https://academic.oup.com/biolinnean/article/100/4/866/2450537},
	author = {Grace, T. and Dowell, F. and Anthony Joern and Wisely, S.M. and Brown, S.J. and Maghirang, E.}
}
@article {KNZ001273,
	title = {Highly polymorphic microsatellites in the North American snakeweed grasshopper, Hesperotettix viridis},
	journal = {Journal of Orthoptera Research},
	volume = {18},
	year = {2009},
	pages = {19 -21},
	abstract = {<p>Microsatellite markers are preferred for fine-scale population genetic studies requiring high resolution. The grasshopper Hesperotettix viridis (Thomas) is an oligophagous species that feeds on composites and often exhibits locally restricted diets. Divergence in host plant use in some localities is seen where co-occurring subpopulations select alternate plant species, as expected with the evolution of host shifts and associated lineage divergence. To characterize the host-associated divergence patterns among populations of H. viridis, we developed markers from two microsatellite-enriched genomic libraries. Here we report the characterization and optimization of seven polymorphic di- and tri-nucleotide microsatellite loci for this species. One hundred and six individuals from 5 populations were tested for polymorphism. The number of alleles varied from 4 to 38 in all the populations. Ho ranged from 0.339 to 0.790. Homozygote excess was observed across loci, perhaps due to inbreeding. This is the first report of microsatellite markers for the subfamily Melanoplinae.</p>},
	keywords = {LTER-KNZ, Hesperotettix viridis, host-associated divergence, local adaptation, microsatellite markers, snakeweed grasshopper},
	doi = {10.1665/034.018.0111},
	url = {https://bioone.org/journals/Journal-of-Orthoptera-Research/volume-18/issue-1/034.018.0111/Highly-Polymorphic-Microsatellites-in-the-North-American-Snakeweed-Grasshopper-iHesperotettix/10.1665/034.018.0111.full},
	author = {Grace, T. and Anthony Joern and Brown, S.J. and Apple, J.L. and Wisely, S.M.}
}
