@article {KNZ001334,
	title = {Comparative genome scan detects host-related divergent selection in the grasshopper Hesperotettix viridis},
	journal = {Molecular Ecology},
	volume = {19},
	year = {2010},
	pages = {4012 -4028},
	abstract = {<p>In this study, we used a comparative genome scan to examine patterns of population differentiation with respect to host plant use in Hesperotettix viridis, a Nearctic oligophagous grasshopper locally specialized on various Asteraceae including Solidago, Gutierrezia, and Ericameria. We identified amplified fragment length polymorphism (AFLP) loci with significantly elevated FST (outlier loci) in multiple different-host and same-host comparisons of populations while controlling for geographic distance. By comparing the number and identities of outlier loci in different-host vs. same-host comparisons, we found evidence of host plant-related divergent selection for some population comparisons (Solidago- vs. Gutierrezia-feeders), while other comparisons (Ericameria- vs. Gutierrezia-feeders) failed to demonstrate a strong role for host association in population differentiation. In comparisons of Solidago- vs. Gutierrezia-feeding populations, a relatively high number of outlier loci observed repeatedly in different-host comparisons (35\% of all outliers and 2.7\% of all 625 AFLP loci) indicated a significant role for host-related selection in contributing to overall genomic differentiation in this grasshopper. Mitochondrial DNA sequence data revealed a star-shaped phylogeny with no host- or geography-related structure, low nucleotide diversity, and high haplotype diversity, suggesting a recent population expansion. mtDNA data do not suggest a long period of isolation in separate glacial refugia but are instead more compatible with a single glacial refugium and more recent divergence in host use. Our study adds to research documenting heterogeneity in differentiation across the genome as a consequence of divergent natural selection, a phenomenon that may occur as part of the process of ecological speciation.</p>},
	keywords = {LTER-KNZ},
	doi = {10.1111/j.1365-294X.2010.04792.x},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-294X.2010.04792.x},
	author = {Apple, J.L. and Grace, T. and Anthony Joern and Amand, P. and Wisely, S.M.}
}
@article {KNZ001273,
	title = {Highly polymorphic microsatellites in the North American snakeweed grasshopper, Hesperotettix viridis},
	journal = {Journal of Orthoptera Research},
	volume = {18},
	year = {2009},
	pages = {19 -21},
	abstract = {<p>Microsatellite markers are preferred for fine-scale population genetic studies requiring high resolution. The grasshopper Hesperotettix viridis (Thomas) is an oligophagous species that feeds on composites and often exhibits locally restricted diets. Divergence in host plant use in some localities is seen where co-occurring subpopulations select alternate plant species, as expected with the evolution of host shifts and associated lineage divergence. To characterize the host-associated divergence patterns among populations of H. viridis, we developed markers from two microsatellite-enriched genomic libraries. Here we report the characterization and optimization of seven polymorphic di- and tri-nucleotide microsatellite loci for this species. One hundred and six individuals from 5 populations were tested for polymorphism. The number of alleles varied from 4 to 38 in all the populations. Ho ranged from 0.339 to 0.790. Homozygote excess was observed across loci, perhaps due to inbreeding. This is the first report of microsatellite markers for the subfamily Melanoplinae.</p>},
	keywords = {LTER-KNZ, Hesperotettix viridis, host-associated divergence, local adaptation, microsatellite markers, snakeweed grasshopper},
	doi = {10.1665/034.018.0111},
	url = {https://bioone.org/journals/Journal-of-Orthoptera-Research/volume-18/issue-1/034.018.0111/Highly-Polymorphic-Microsatellites-in-the-North-American-Snakeweed-Grasshopper-iHesperotettix/10.1665/034.018.0111.full},
	author = {Grace, T. and Anthony Joern and Brown, S.J. and Apple, J.L. and Wisely, S.M.}
}
